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The reversal potential (near 0 mV) of the EPSP indicates that glutamate opens receptors selectively permeable to cations (Na + , K + , and Ca 2+ )

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At the time of incision (0 h), after 24 and 48 h of incubation with treatments, three photos per well were acquired under an optical microscope (Olympus IX51 model) at a magnification of 4